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KMID : 0380420130370040153
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Journal of Prventive Veterinary Medicine
2013 Volume.37 No. 4 p.153 ~ p.162
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Whole genome-scale transcriptome analysis of the amyristolyated enterohemorrhagic Escherichia coli O157:H7
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Yoon Jang-W.
Kwon Ka-Hee
Park Yong-Ho
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Abstract
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Previously, we demonstrated the presence of a second copy of LPS myristoyl transferase in enterohemorrhagicEscherichia coli O157:H7, an important zoonotic diarrheagenic food-borne pathogen; the pO157-encoded ecf (an eae-conservedfragment) and the chromosomally-encoded lpxM (also referred to as msbM) genes. Although both genes share the samefunction as an LPS myristoyl transferase, the pO157-encoded ecf is thermoregulated via an intrinsically curved DNA whilethe chromosomal lpxM is regulated by the PhoP/Q two component regulatory system. However, it is unclear why E. coli O157:H7 carries two copies of LPS myristoyl transferase that are differentially regulated. In this study, a whole genome-scaletranscriptome specific to E. coli O157:H7 was carried out for identification of the genes differentially expressed in theamyristoylated E. coli O157:H7. The results identified a total of 110 EHEC genes that were up- or down-regulated in theamyristoylated E. coli O157:H7 strain, including genes associated with virulence (26.36%), metabolism (20.91%), transport(10.91%), signal transduction (4.55%), genetic information processing (3.64%), stress response (2.73%), regulatory function(2.73%), motility/adherence (3.64%), cell envelope (2.73%), cell division (1.82%) and ORFs of unknown function (17.27%). Of particular interest, the expression of LEE pathogenicity island genes was significantly influenced by LPS structural defects.
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KEYWORD
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Whole genomic transcriptomics, amyristolyation, enterohemorrhagic, Escherichia coli O157, H7
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